Experimental and Evidence based Protocols in Pharmacology provides a holistic coverage of scientific protocols involved in the pharmacology and toxicological studies. The book serves as a reference to the biomedical researchers working in pharmacology and allied subjects to mitigate the challenges in the routine laboratory procedures. Book has 22 chapters with evidence based protocols and procedures to conduct the defined experiment. Further, this book provides single reference window with evidence based practical approaches to cater the need of academician and research scholars. The book is designed in a diligent manner to provide majority of protocols viz. Primary cell culture, Mesenchymal stem cells isolation and its application, DNA fragmentation Assay, oxidative stress evaluation protocols, Cytotoxicity and Genotoxicity Assays, Apoptosis studies, Analytical studies involving HPLC, GC-MS, ICP-OES and experimental pharmacological tool in smooth muscle pharmacological studies etc. to cement the scientific acumen.

Experimental and Evidence based Protocols in Pharmacology provides a holistic coverage of scientific protocols involved in the pharmacology and toxicological studies. The book serves as a reference to the biomedical researchers working in pharmacology and allied subjects to mitigate the challenges in the routine laboratory procedures. Book has 22 chapters with evidence based protocols and procedures to conduct the defined experiment. Further, this book provides single reference window with evidence based practical approaches to cater the need of academician and research scholars. The book is designed in a diligent manner to provide majority of protocols viz. Primary cell culture, Mesenchymal stem cells isolation and its application, DNA fragmentation Assay, oxidative stress evaluation protocols, Cytotoxicity and Genotoxicity Assays, Apoptosis studies, Analytical studies involving HPLC, GC-MS, ICP-OES and experimental pharmacological tool in smooth muscle pharmacological studies etc. to cement the scientific acumen. This book imbibes all the basic and advanced research protocols along with their translational approaches in experiments involved in pharmacology and also provides the insight of example based drug assays to help in bioprospecting of naive molecules.

Further, this book also provide a reference book to the researchers involved in toxicological testing using OECD testing guideline to calculate the lethal dose of any compound in a better manner. This MS also suffice the different methods to calculate the median lethal dose (LD50). The important reproductive toxicity assays to ascertain the toxic potential of any xenobiotics is also incorporated in this MS. Moreover, sophisticated technique like Flow cytometry and other experimental models viz. Direct and Indirect LPS induced acute lung injury model has also well written to provide an insight of the experimentation involving laboratory animals.

Content

1 Preparation of Primary Cell Cultures from Different Tissues/Organs

Atul Prakash, Rajesh Mandil, Amit Shukla and A.K. Srivastava

2 Procedures to Evaluate Cytotoxicity in Primary Cell Culture

Atul Prakash, Rajesh Mandil, Amit Shukla and A.K. Srivastava

3 Genotoxicity Assays for Safety Evaluation of Chemicals inCell Culture

Atul Prakash, Rajesh Mandil and A.K. Srivastava

4 DNA Fragmentation Assay

Rajesh Mandil, Atul Prakash, S. P. Singh and Preeti Singh

5 Single Cell Gel Electrophoresis (SCGE/Comet assay) inPrimary Cell Culture

Atul Prakash and Rajesh Mandil

6 Micronuclei Induction Assay in Primary Cell Culture

Atul Prakash and Rajesh Mandil

7 Protocol for TUNEL Assay in Primary Cell Culture

Rajesh Mandil and Atul Prakash

8 Screening of Myometrial Relaxants in ExperimentalDysmenorrhoea an in vitro Model

Afroz Jahan, M. K. Lonare, Sanweer Khatoon and G. S. Rao

9 Direct and Indirect LPS Induced Acute Lung Injury Model

Meemansha Sharma, Anshuk Sharma, Thakur Uttam Singh

10 A Laboratory Protocol for Mesenchymal Stem Cell Isolationfrom Veterinary Important Species

Milindmitra K Lonare, Afroz Jahan, Manjinder Sharma, andSivaraman Ramanarayanan

11 Detection of Environmental Pollutants and AgrochemicalsUsing Gas Chromatography and Mass Spectrophotometry

Amit Shukla, Soumen Choudhury, Atul Prakash, Sakshi Tiwari

12 Basics of Flow Cytometric Analysis

Amit Shukla, Mukul Anand, Soumen Choudhury, Sakshi Tiwari

13 Determination of Aflatoxin Using HPLC

Shalini Vaswani, Mukul Anand

14 ICP-OES to Estimate Minerals in Biological Samples

Shalini Vaswani, Mukul Anand

15 Recording of Myometrial Reactivity Using Polyphysiograph

Soumen Choudhury, Amit Shukla, Preeti Singh and Atul Prakash

16 Estimation of Oxidative Stress in Hepatic Tissue

Amit Shukla and Sakshi Tiwari

17 Drug Metabolizing Enzymes Assay

Atul Prakash and Amit Shukla

18 Acetylcholine Esterase (AChE) Assay

Atul Prakash and Amit Shukla

19 Preparation of Hepatocyte Culture for Toxicity Testing ofXenobiotics

S.P. Singh and Atul Prakash

20 Detection of Apoptosis by DNA Gel Electrophoresis

Atul Prakash and Amit Shukla

21 Lethal Dose 50 Calculation Methods

Amit Shukla, Atul Prakash, Soumen Choudhury and Sakshi Tiwari

22 OECD Guidelines for Acute Animal Toxicity Tests

Amit Shukla and Sakshi Tiwari

23 Evaluation of Reproductive Toxicity Potential of Xenobiotics

Atul Prakash and Amit Shukla