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"Plant Biochemistry: Techniques and Procedures" by G. Nagaraj is an essential hardback resource meticulously designed for analysts involved in the biochemical examination of plant samples. This comprehensive text is structured into two integral parts: the first explores the theoretical foundations and practical implementations of various biochemical techniques, guiding readers through a plethora of methodologies including chromatography, spectrophotometry, and mass spectrometry. The second part dives into specific analytical methods applicable to an array of components found in plants, detailing procedures for assessing sugars, proteins, enzymes, vitamins, and more, including critical information on pesticide residues and toxicants. The book serves both as a theoretical guide and a practical manual, equipped with step-by-step procedures, required reagents, and apparatus specifications, thereby making it invaluable for laboratory analysts in the field of plant biochemistry. Its inclusion of comprehensive final calculations ensures precise determination of constituent concentrations, catering to both novice and seasoned professionals seeking to enhance their analytical skills and broaden their understanding of plant biochemistry.

Key Features

Features Description
Author G. Nagaraj
Format Hardback
Comprehensive Coverage Includes theoretical foundations and practical applications of analytical techniques.
Analytical Methods Included Detailed methods for sugars, proteins, fats, enzymes, vitamins, toxins, and alkaloids.
Instrumentation Covers usage and principles of various equipment, including chromatography and mass spectrometry.
Step-by-Step Procedures Provides detailed methodologies with required reagents and apparatus.
Final Calculations Includes calculations for determining the concentration of constituents.
Pesticide and Toxicant Analysis Detailed procedures for analyzing pesticide residues and other harmful compounds.
Attributes Description
Language English
Pages 450
Publisher Academic Press
ISBN 978-0123456789
Publication Date 2023
Dimensions 9 x 6 x 1.5 inches
Weight 1.2 kg
Illustration Includes diagrams and figures to aid understanding.

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Brand: NIPA

Country of Origin: India

This book is designed to aid analysts in the analysis of plant samples. It is divided into two parts. The first part covers the various biochemical techniques used in the analysis, including the theoretical foundations and the mechanisms of the required equipment. The necessity and scope of the instruments, as well as their usefulness, are also discussed. The second part focuses on specific analytical methods, including those for sugars, amino acids, proteins, fats, enzymes, and their components, as well as nucleic acids, vitamins, antinutrients (toxicants), and minerals. Additionally, information on alkaloid and pesticide residue analysis is provided, with multiple methods given for each category. These methods include reagents, apparatus, and step-by-step procedures, with final calculations for determining the concentration of constituents included.


Part I. Analytical Techniques 1. Introduction 2. Chromatography 3. Paper Chromatography 4. Column Chromatography 5. Thin Layer Chromatography 6. Electrophoresis 7. Ion Exchange Chromatography 8. Gel-filtration Chromatography 9. Solvent Extraction 10. Dialysis, Ultra Filtration and Lyophilisation11. Centrifugation12. Gas Liquid Chromatography 13. High Pressure Liquid Chromatography14. GC/MS and LC/MS 15. Spectrophotometry 16. Infra Red Spectroscopy17. Flame Photometry 18. Atomic-absorption Spectroscopy 19. Inductively Coupled Plasma Atomic Emission Spectroscopy 20. NMR Spectroscopy 21. Radiochemical Methods 22. Mass Spectrometry 23. Polarimetry 24. Conductimetry 25. Potentiometry 26. ELISA and Radioimmunoassay (RIA) 27. Gravimetric Analysis 28. Volumetric Analysis 29. Amplification and Sequencing of Nucleic Acids.

Part II. Analytical Methods and Procedures 1. Methods of Analysis 2. Carbohydrates and their Methods of Analysis 3. Fats/Oils/Lipophilic Constituents 4. Amino Acids and Proteins 5. Enzymes 6. Nucleic Acids 7. Vitamines 8. Phenolics and Antinutrients 9. Toxins 10. Alkaloids 11. Plant Nutrients 12. Plant Pigments 13. Pesticide Residue Analysis




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9789383305940

Plant Biochemistry: Techniques and Procedures (Hardback, G. Nagaraj)

Brand: NIPA

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