The book Handbook on Basic Laboratory Skills is designed to serve two primary goals: (i) for teaching basic concepts of laboratory techniques and (ii) following good laboratory practices while working in laboratory or doing research. The book presents the importance of laboratory skills/practices and biosafety issues to students and researchers. The chapters included in this book cover information on handling of laboratory chemicals, equipment, preparation of buffers, media, basics of seed testing, biosafety, etc. which constitute curriculum of the course Basic Concepts in Laboratory Techniques (PGS504, 0L + 1P) for Post-Graduate students of the Indian National Agricultural Research System (NARS) including Deemed Universities, State Agricultural Universities (SAUs), and Central Agricultural Universities (CAUs).

The book Handbook on Basic Laboratory Skills is designed to serve two primary goals: (i) for teaching basic concepts of laboratory techniques and (ii) following good laboratory practices while working in laboratory or doing research. The book presents the importance of laboratory skills/practices and biosafety issues to students and researchers. The chapters included in this book cover information on handling of laboratory chemicals, equipment, preparation of buffers, media, basics of seed testing, biosafety, etc. which constitute curriculum of the course Basic Concepts in Laboratory Techniques (PGS504, 0L + 1P) for Post-Graduate students of the Indian National Agricultural Research System (NARS) including Deemed Universities, State Agricultural Universities (SAUs), and Central Agricultural Universities (CAUs).

Content

 Handling of Chemicals and Solutions in Laboratory

 Drying Chemicals and Solutions in Laboratory

 Washing, Drying and Sterilization of Laboratory Glassware

 Use and Handling of Specific Glassware in Laboratory

 Use and Handling of Laboratory Equipment

 Use and Handling of Vacupette and Micropipette

 Use and handling of Microscopes

 Weighing of Chemicals and Preparation of Solutions

 Preparation of Different Agrochemicals

 Measurement of pH and Preparation of Buffer

 Handling of Acid/Base and Titration in Laboratory

 Preparation of Medium and Sterilization

 Plant Tissue Culture

 Testing of Seed Viability

 Testing of Pollen Viability

 Description of Flowering Plants

 Handling of Hazardous Materials

 Electrical Wiring and Earthing in Laboratory

 Safety Measures in Laboratory

 Biosafety and Ethical Issues of Laboratory Research

 Do and Don’ts in a Laboratory